How to Order Your Aptamers?

OTC Biotech has developed DNA aptamers against a broad array of small molecule and whole cell targets. The highest affinity and most specific aptamers are listed for each category. In some cases binding between the top aptamer choices was virtually identical. Therefore, all of the top aptamer candidates are offered for sale. Researchers are encouraged to purchase and try any or all of the candidate aptamers for their particular applications. Applications can include ELISA-like or “ELASA” assays, aptamer “Western” blotting, and cell or tissue staining. Potential users are encouraged to read the reference list below which contain suggested protocols.

Aptamers

Suggested Protocol References:

ELISA-like or “ELASA” assays References:

All OTC Biotech DNA Aptamers are available in unmodified form for $200 each (> 1 mg of desalted 55-72 base DNA) with a choice of 5’-primary amine, biotin or fluorescein (FAM) groups for an additional charge of $60. Other custom modifications, fluorophores, or purifications (HPLC or PAGE) are possible, but the customer should call OTC Biotech at 210-731-0015, ext. 2234 to discuss such modifications. Each aptamer can be rehydrated in standard PBS (pH 7.2-7.4) or other neutral aqueous buffers for use in most applications.

Bruno J.G., Carrillo M.P., Phillips T., Edge A. Discrimination of recombinant from natural human growth hormone using DNA aptamers. J. Biomolec. Techniques. 22:27-36, 2011.

Bruno J.G., Carrillo M.P., Cadieux C.L., Lenz D.L., Cerasoli D.M., Phillips T. DNA aptamers developed against a soman derivative cross-react with methylphosphonic acid but not with the flanking hydrophobic groups. J. Molecular Recognition. 22:197-204, 2009.

Vivekananda J., Kiel J.L. Anti-Francisella tularensis DNA aptamers detect tularemia antigen from different subspecies by Aptamer-Linked Immobilized Sorbent Assay. Lab Invest. 86:610-618, 2006.

Aptamer “Western” Blotting

Ramos E, Piñeiro D, Soto M, Abanades DR, Martín ME, Salinas M, González VM. A DNA aptamer population specifically detects Leishmania infantum H2A antigen. Lab Invest. 87:409-416, 2007.

Shin S, Kim IH, Kang W, Yang JK, Hah SS. An alternative to Western blot analysis using RNA aptamer-functionalized quantum dots. Bioorg. Med. Chem. Lett. 20:3322-3325, 2010.

Aptamer Cytochemical and Histochemical Staining

Bruno J.G., Carrillo M.P., Phillips T., King B. Development of DNA aptamers for cytochemical detection of acetylcholine. In Vitro Cell. Develop. Biol. – Animal. 44:63-72, 2008.

Bruno J.G., Savage A., Carrillo M.P., Richarte A.M., Phillips T., Edge A., Koke J.R. Anti-N-acetyl-glucosamine DNA aptamers bind chitin on Penicillium cell walls to enable fluorescent and gold staining microscopy techniques. J. Bionanosci. 4:45-52, 2010.

Gupta S, Thirstrup D, Jarvis TC, Schneider DJ, Wilcox SK, Carter J, Zhang C, Gelinas A, Weiss A, Janjic N, Baird GS. Rapid histochemistry using slow off-rate modified aptamers with anionic competition. Appl. Immunohistochem. Mol. Morphol. 19:273-278, 2011.